WPU BioChemistry

• 1. Introduction to Biochemistry

• General Biochemistry Overview

  • Introduction to Biochemistry
  • Introduction to Biochemistry (More In-Depth)

  Metabolism Overview

  • Metabolism and ATP

  Biomolecules Overview

  • Biomolecules
  • Macromolecules Overview

• 2. Life at the Molecular Level

• Thermodynamics Overview

  • The Laws of Thermodynamics, Entropy, and Gibbs Free Energy

  Water and Its Properties

  • Properties of Water and Hydrophobic Effect
  • Properties of Water
  • Water as the “Universal Solvent”

  Chemical Bonds

  • 4 Types of Chemical Bonds
  • Types of Noncovalent Interactions

  Acids, Bases, & Buffers

  • Acids and Bases, pH and pOH
  • Acid-Base Equilibria and Buffer Solutions (Henderson-Hasselbalch)
  • Henderson-Hasselbalch Practice Problem
  • Physiologic pH and Buffers
  • Acid-Base Titration
  • Buffers and Titration Curves

• 3. Amino Acids, Peptides, and Proteins

• Proteins

  • Proteins Overview

  Amino Acids

  • Introduction to Amino Acids
  • Introduction to Amino Acids #2
  • Amino Acids Grouping
  • Nonpolar and Uncharged Polar Amino Acids
  • Basic and Acidic Amino Acids
  • Amino Acid Titration Curves & Isoelectric Point (pI)
  • Ionization of Amino Acids
  • Amino Acid Titration
  • Calculating Isoelectric Point of Proteins (Example)

  Peptides

  • Peptide Bond Formation

  Protein Purification & Analysis

  • Protein Isolation & Protein Analysis Overview
  • Isoelectric Point and Electrophoresis
  • SDS-Polyacrylamide Gel Electrophoresis
  • Salting Out Proteins
  • Ion Exchange Chromatography
  • Gel Filtration Chromatography
  • Affinity Chromatography
  • Analysis of Protein Purification
  • Analysis of Protein Purification (Part II)

  Isoelectric Focusing and Isoelectric Point

  • Isoelectric Focusing and Isoelectric Point
  • Isoelectric Focusing and Isoelectric Point (Part II)

• 4. Protein Structure and Function

• Protein Structure and Folding

  • Protein Structure #1
  • Protein Structure #2
  • Protein Structure #3

  Dihedral Angles and Ramachandran Plots

  • Ramachandran Plot Overview
  • Phi and Psi Angles
  • Ramachandran Principle: Protein Atomic Clashes vs Phi & Psi

  Fibrous vs Globular Proteins

  • Comparing Fibrous and Globular Proteins

  Myoglobin & Hemoglobin

  • Hemoglobin and Myoglobin
  • Oxygen Binding Curve for Myoglobin and Hemoglobin
  • T-State and R-State of Hemoglobin

  Enzymes

  • Enzymes Review
  • Enzymes #1
  • Enzymes #2
  • Enzyme Kinetics - Michaelis-Menten Curve
  • Michaelis-Menten Equation
  • Lineweaver-Burk Plot
  • Enzyme Cooperativity
  • Enzyme Cooperativity & Hill’s Coefficient
  • Enzyme Inhibition

  Serine Proteases

  • Serine Protease Background & Catalytic Mechanism
  • Serine Protease Mechanism

• 5. Carbohydrates and Glycobiology

• Introduction to Carbohydrates & Overview

  • Introduction to Carbohydrates
  • Carbohydrates Overview

  Carbohydrates - Sugars and Monosaccharides Explained

  • Carbohydrates: Simple Sugars and Fischer Projections
  • Biochemistry of Carbohydrates
  • Nomenclature and Stereochemistry of Carbohydrates
  • Aldoses, Ketoses, Fischer Projections, and Epimers
  • Cyclic Form of Carbohydrates

  Disaccharides & Polysaccharides

  • Disaccharides (Maltose, Lactose, and Sucrose)
  • Polysaccharides Explained
  • Polysaccharides (Glycogen, Starch, & Cellulose)

  Glycobiology

  • Glycosaminoglycans & Proteoglycans
  • Glycosylation & Glycoproteins
  • Glycolipids

• 6. Nucleotides and Nucleic Acids; Genomics

• Nucleic Acids and Nucleotides

  • Nucleic Acids Overview
  • Composition of Nucleic Acids
  • Nucleosides and Nucleotides
  • DNA - Structure & Function - Nucleosides & Nucleotides
  • Purines, Pyrimidines, DNA, RNA, Denaturation, and Annealing
  • Types of RNA

  Genomics

  • Recombinant DNA Technology

• 7. Lipids, Membranes, Transport, and Bio-Signaling

• Lipids

  • What are Lipids?
  • Types of Lipids
  • Lipids Overview
  • Lipid Biochemistry
  • Steroids
  • Triglycerides

  Fatty Acids

  • Fatty Acids
  • Fatty Acids (Part 2)

  Terpenes

  • Terpenes #1
  • Terpenes #2

  Membranes & Membrane Lipids

  • Types of Membrane Lipids
  • Phospholipids
  • Micelles and Lipid Bilayer
  • Liposomes (Lipid Vesicles)
  • Cholesterol and Fatty Acids Regulate Membrane Fluidity

  Cell Membrane Structure & Functions

  • Cell Membrane Structure & Functions
  • Properties of Cell Membranes

  Bio-Signaling

  • Receptors: Signal Transduction and Phosphorylation Cascade
  • Hormones & Signal Transduction
  • Common Cell Signaling Pathways

• 8. Introduction to Catabolism and Metabolism

• Introduction to Metabolism

  • Introduction to Metabolism

  Introduction to Metabolic Pathways

  • Introduction to Metabolic Pathways

  Introduction to Glucose Metabolism

  • Introduction to Glucose Metabolism

  Bioenergetics

  • Bioenergetics

  Bioenergetics and Regulation of Metabolism (In-Depth) #1

  • Bioenergetics and Regulation of Metabolism (In-Depth) #1

  Bioenergetics and Regulation of Metabolism (In-Depth) #2

  • Bioenergetics and Regulation of Metabolism (In-Depth) #2

• 9. Glycolysis and Gluconeogenesis

• Glycolysis

  • Glycolysis Overview
  • Glycolysis Steps
  • Glycolysis Steps Walkthrough

  Gluconeogenesis

  • Introduction to Gluconeogenesis
  • Gluconeogenesis Steps Walkthrough

• 10. Regulation of Protein/Enzyme Function

• Reciprocal Regulation of Gluconeogenesis and Glycolysis

  • Reciprocal Regulation of Gluconeogenesis and Glycolysis

  Positive & Negative Feedback Regulation of Enzymes

  • Positive & Negative Feedback Regulation of Enzymes

  Enzyme Regulation

  • Enzyme Regulation

  cAMP - ATP, ADP, cAMP

  • cAMP - ATP, ADP, cAMP

• 11. Metabolic Pathways

• Every Major Metabolic Pathway Explained

  • Every Major Metabolic Pathway Explained

  Citric Acid Cycle

  • Introduction to Citric Acid Cycle
  • Citric Acid Cycle Explained
  • Citric Acid Cycle Steps Overview

  Oxidative Phosphorylation

  • Introduction to Oxidative Phosphorylation
  • The Electron Transport Chain and Oxidative Phosphorylation

  Introduction to Fatty Acid Metabolism

  • Introduction to Fatty Acid Metabolism

  Fatty Acid Synthesis

  • Introduction to Fatty Acid Synthesis
  • Fatty Acid Synthesis Overview (Part 1)
  • Fatty Acid Synthesis Overview (Part 2)
  • Fatty Acid Synthesis Mechanism Walkthrough

  Fatty Acid Breakdown (Beta-Oxidation)

  • Oxidation of Fatty Acids
  • Beta Oxidation Walkthrough

  Pentose Phosphate Pathway (PPP)

  • Introduction to Pentose Phosphate Pathway
  • Pentose Phosphate Pathway Walkthrough

  Glycogen Metabolism

  • Glycogen Metabolism Overview
  • Glycogen Synthesis
  • Glycogen Breakdown

  Amino Acid Metabolism

  • Amino Acid Metabolism Overview
  • Introduction to Amino Acid Metabolism
  • Amino Acid Metabolism
  • Glucogenic and Ketogenic Amino Acids

  Urea Cycle

  • Urea Cycle